Bacterial & Fungal Assays

MEDINA develops different phenotypic and target-based assays, using enzymatic and whole cell approaches, both on-demand and optimized tests.

Custom development from your tube assay to a microtiter plate (96/384) assay for High-Throughput Screening (HTS), hit confirmation, hit-to-lead and Mechanism of Action (MoA) of the lead.


Bacterial & Fungal Assays

  • Fungal synergy (Candida, yeast; Aspergillus, fungi)
  • Bacterial synergy (Gram positive/negative)
  • Panel human pathogens (Wild type/multidrug resistant/nocosomial strains Gram- & Gram+/yeast/fungi)
  • Panel yeast pathogens (six Candida spp)
  • Tuberculosis panel of surrogate strains (Mycobacterium tuberculosis H37ra /Mycobacterium bovis)
  • Biofilms (Wild type/multidrug resistant Acinetobacter baumannii; Salmonella typhimurium; Wild type/multiresistant MRSA...)
  • LpxC (Gram negative-A. baumannii, P. aeruginosa, E. coli)
  • Reactive oxygen species (ROS): whole cell ( E. coli)
  • Oral pathogens: mutans, L. acidophilus, P. gingivalis
  • Absorbance
  • Fluorescence: fluorescence intensity (FI)
  • Luminiscence: glow, flash, dual luminescence
  • Imaging-halo Inhibition zone (Solid Agar Assays)
  • LC-MS/MS and LC/HRMS
  • Liquid Handling Systems (from 1 µl to 1 ml) with 96- and 384-pipetting heads (Freedom EVO, Tecan; Biomek FX and i7, Beckman; EP3, PerkinElmer)
  • Echo® Acoustic Liquid Handling System (from 15 nl to 1 µl), Beckman
  • Multidrop Combi System (from 0.5 µl to 2.5 ml) for 96- and 384-well plates (ThermoFisher)
  • Multiplate reading system (Envision & Victor-2, PerkinElmer)
  • In-house Image Analyzer
  • Flow cytometry (BD Accuri C6, Becton Dickinson)
  • Mass spectrometry (Api4000 (Sciex) and QTOF5600 (Sciex))
  • HPLC-DAD (Agilent 1290 DAD)

Quality control: We perform QC of each screening plate automatically on signal/background ratio and Z´ score calculations, considering the best plate layout for your assay; a statistically optimal number of repeats are performed for the internal positive and negative controls, and/or a dose-response curve of relevant pharmacological standards when available. Genedata Screener® software and in-house applications help us perform the analysis, visualizations, and management of screening data.