MEDINA develops gene editing projects using the revolutionary CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 technology, which allows the introduction of mutations in targeted areas of human genes, including indels (insertion/deletion) or single point mutations.
CRISPR-based gene editing
- sgRNA design: We design the sgRNA and oligonucleotide donors to knock-out or knock-in human genes.
- CRISPR-Cas9 editing: Generation of human cells harboring the desired mutations in one or two alleles.
- Human cell lines (Propietary cell lines or cell lines provided by the user)
- iPSC (Propietary iPSC lines for research only, or cell lines provided by the user)
- Clonal cell preparation
- Clonal cell expansion and characterization
- Sequence validation
- Expression profiling (on demand)
- qRT-PCR, WB, SDS-PAGE
- Cell maintenance, expansion and cryopreservation
- Electroporation
- FACS Sorting
- Limiting dilution
- Fluorescence
