The Chemistry Department offers its expertise in natural products research to solve problems associated to the identification and purification of natural products. The experience of our team in international industrial research allows the parallel handling of large numbers of complex microbial extracts for the discovery of new Natural Products in a highly efficient, cost-effective manner.
Natural Products Chemistry
- LC/MS based metabolomic analysis of samples for the identification of biomarkers associated to changes in composition of natural extracts.
- Dereplication of components in extracts and fractions using LC/HRMS and internal and commercial databases
- Dereplication of compounds in pure or semi-purified fractions by NMR.
- Solvent extraction of microbial cultures or natural samples.
- Ad-hoc development of protocols for the extraction of selected components in a natural product extracts or mixture
- Solid phase extraction (SPE) of culture broths of microorganisms
- Chromatographic fractionation of extracts for the isolation of natural products of interest
- Wide range of approaches including the use of normal and reverse phase chromatography, gel filtration and polystirenic resins
- HPLC purification as final step of the process.
- HPLC separation of extracts or complex mixtures of molecules
- Wide range of columns and chromatographic adsorbents
- Semi-preparative and preparative columns for the separation of variable amounts of sample.
- Acquisition of HRMS and NMR experiments necessary for the structural elucidation of new molecules
- Interpretation of spectra
- Structural elucidation of complex molecules isolated even in submilligram quantities.